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Description

The medium was IMDM + 10% FCS (fetal calf serum)

Watch for the background materials

When taking a B vitamin supplement, follow the product label or the directions of your healthcare provider

The slides were then stained with primary antibody (in PBS, 45 min), washed with PBS (15 min) and incubated in the dark with Alexa 488-labelled second antibody (1:1,000, 45 min.), or with Cy3-labelled secondary antibodies (1:1,000, 45 min)