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After a 20-min incubation with immunol staining blocking buffer (Beyotime, Shanghai, China), cells were continuously incubated with mouse anti-SMA (1:100, Abcam) and rabbit anti-CD133 (1:100, Abnova) 44 at 4 C overnight

Step 2: Hydrate Consistently

There are two main types of melanin in the human body: eumelanin (which provides darker, brown, or black tones) and pheomelanin (which provides lighter, yellow, or red tones)