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Because vitamin B 12 in supplements is not bound to protein, and because intrinsic factor (IF) is still available, the absorption of supplemental vitamin B 12 is not reduced as it is in pernicious anemia

2018;32(2):114-121.doi:10.1038/s41371-017-0005-2 Zittermann A, Ernst JB, Prokop S, etal

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Before each run, the system was preconditioned with simulated gastric fluid (65 mL, pH 2.0) in the stomach compartment and simulated intestinal fluid (55 mL, pH 7.0) in the intestinal compartment

The guidelines acknowledge that a negative IF-Ab test does not rule out AIG or PA, and they suggest additional investigations when clinical suspicion remains