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References 1 AbdelraheemE.ThairB.VarelaR

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Unlike frost-free freezers, which cycle through temperature fluctuations and could further compromise sample integrity, these storage conditions likely minimized additional variability

Ariga, Physical Chemistry Chemical Physics 15, (2013) 1058010611

However, in the background of the wild-type enzyme no slight increase in basal activity was observed with these mutations 49 , and as such, we believe that altered oligomerisation should be considered as an aspect of rescue