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Blocking and permeabilization were performed for 1 h at room temperature in PBS containing 2% BSA and 0.1%, 0.2%, 0.5% or 1% Triton X-100 depending on the antigen (Supplementary Data 5)

Mar Biotechnol 24:9911001 Usharani G, Srinivasan G, Sivasakthi S, Saranraj P (2015) Antimicrobial activity of spirulina platensis solvent extracts against pathogenic bacteria and fungi Department of Civil and Structural Engineering, Annamalai University