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At least 10 cells (membrane sheets) per experiment ( n = 3) were imaged, and ten ROIs (10 10 m each) were selected from different regions of each image for dot density quantification after deconvolution (DeconvolutionLab2 in ImageJ)

This effect is blocked by glutamatergic antagonist CNQX [cyanquixaline (6-cyano-7-nitroquinoxaline-2,3-dione)] and AP5 (2-amino-5-phosphopentanoic acid), suggesting that interneuron-astrocyte-mediated potentiated inhibition of pyramidal neurons depends on astrocyte-mediated glutamate release (Kang et al., 1998)