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This culture was then diluted into 30 mL of YPD medium to give an initial OD 600 of 1.0 and incubated at 30 C in the rotary shaker for 4 h

Because strong mitogenic signaling from RAS oncogenes produces ROS [46], which promote cell proliferation, it is possible that cancer cells with an RAS oncogene may be more susceptible to erastin [1, 2], leading to the suppression of GSH synthesis [47]

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Statistics GraphPad Prism 9 (GraphPad Software Inc., San Diego, CA, USA) was used for the data processing and analysis

"The overall thought here is that getting towards one vaccine composition for everyone will ultimately be much, much more helpful," said Dr