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Inhibiting EGLNs, which link glutaminolysis to mTORC1 activation, could effectively inhibit mTORC1 activity

We propose a potential indirect mechanism via which opioid antagonism may be reducing acute ketamine-induced glutamatergic activity

Determination of cell death by PI staining Cells were trypsinized, washed with PBS and suspended in 300 l Dulbeccos phosphate buffered saline (DPBS) supplemented with 0.2% BSA containing 0.3 g/ml propidium iodine (PI, Sigma-Aldrich)