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Nguyen, P.H

Factor in waste, which typically runs 5 to 10% due to vial dead space and measurement imprecision

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Finally, the coverslips were incubated for 1 hour in a humidified chamber at RT in secondary Abs (1:200, unconjugated from Jackson ImmunoResearch and conjugated in house as previously described (Coons et al., 1942), donkey anti-mouse IgG: cat# 715-005-151, RRID: AB_2340759