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Hughson AL, Hannon G, Salama NA, Vrooman TG, Stockwell CA, Mills BN, Garrett-Larsen J, Qiu H, Katerji R, Benoodt L, Johnston CJ, Murphy JD, Kruger E, Ye J, Gavras NW, Keeley DC, Qin SS, Lesch ML, Muhitch JB, Love TMT, Calvi LM, Lord EM, Luheshi N, Elyes J, Linehan DC, Gerber SA Molecular therapy

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An oligonucleotide with length between 28 and 38 bases was designed to target each codon in the CBS ORF, such that the targeted codon is replaced with an NNK-degenerate codon (a mixture of all four nucleotides in the first and second codon positions, and a mixture of G and T in the third position) using the PopCode oligo suite webtool [11]

This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation