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or an N-oxide, crystalline form, hydrate, or pharmaceutically acceptable salt thereof, with the proviso that: when R 3 is ethyl, R 1 and R 2 cannot be both H, O, or when R 3 is ethyl and at least one of R 1 or R 2 is H, R 1 or R 2 cannot be As used herein, the terms modulate, modulating, modulator and grammatical variations thereof mean to change, such as decreasing or reducing the occurrence of ferroptosis

Paraformaldehyde at a concentration of 4% v/v (pH 7.4) was used to fix the cells for 10 min in the dark after being washed with phosphate-buffered saline (PBS, pH 7.4) 3 times

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On the other hand, it exerted anti-autophagy function by activating mTOR and antagonized autophagy-related ferroptosis mediated by Nrf2/GPX4 (Fig