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After incubation, the cells were quickly washed with phosphate buffered saline (PBS, 0.1 M, pH 7.4), harvested, centrifuged, and each pellet was dispersed in PBS, and then sonicated

doi: 10.1038/s41598-018-28376-z

10.14814/phy2.12751 38 RazaH.PrabuS

A new predictor of toxicity following acetaminophen overdose based on pretreatment exposure

The role of the innate immune system has recently come into play as well because investigators reported an upregulation in Toll-like receptor 4 signaling pathways in animal models of osteonecrosis, leading to activation of osteoclasts.103, 104 TABLE 103-4