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The NOAEL for both systemic toxicity in the parental generation and developmental toxicity in the pups was 200 ppm (equivalent to 10 mg/kg bw per day)

[5] Practical Considerations for Clinical Use Anyone considering BPC-157 as part of a health or longevity protocol should approach it with the same framework applied to any emerging therapy: define the target outcome, select the administration route supported by the best evidence for that target, start at a conservative dose, and monitor response systematically rather than anecdotally

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Cells were then resuspended in 500 L of free HBSS, strained through a 40 m cell strainer (BD Falcon), and analyzed using a flow cytometer (BD LSRFortessa Cell Analyzer, BD Biosciences) equipped with 488 nm laser for excitation