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In one example, the T7 RNA polymerase-promoter system is used and is induced from the lac operon in BL21 (DE3)-based strains to circumvent the need to separately purify and supplement T7 RNA polymerase into CFE reactions

jejuni (Cj) to a CFE reaction to glycosylate a cell-free synthesized protein containing a glycosylation tag

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The labeled protein concentrations were adjusted to 100 nM based on fluorescence intensity measured during pretest scans, ensuring signal quality and uniformity across all three Syn constructs for direct comparison of binding affinities