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One-step reverse transcription-qPCR (real-time PCR) was performed in accordance with methods described by the manufacturer and previous publications [46,47]

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Fixed liver tissue samples were dehydrated in alcohol, embedded in paraffin and stained with hematoxylin and eosin (HE), Massons trichrome, and picrosirius red (PSR)

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Melatonin Melatonin is an amphiphilic molecule that has been identified as a potent antioxidant therapeutic agent in neurodegenerative diseases (Polimeni et al., 2014) associated with mitochondrial dysfunction (Ganie et al., 2016) (Table 1)