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IL-6, IFN-, and TNF were quantified in the supernatants using an MSD immunoassay (V-PLEX proinflammatory kit

These studies on the models have elucidated how this immune-mediated activation of the thyroid gland leads to systemic effects, such as the presence of goiter or the clinical symptoms of hyperthyroidism, which are weight loss, increased heart rate, and nervousness (Wmeau et al