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AAV-mediated SIRT1 overexpression protects against NIHL and hair cell loss To further verify the causal relationship between SIRT1 and NIHL, wild-type C57BL/6 J mice were transfected with AAV2/anc80L65 carrying SIRT1-expressing plasmids to overexpress SIRT1 (AAV-SIRT1) and an AAV vehicle as a negative control (AAV-CTRL) (Fig

Cell 2001;105:683-94

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Techniques such as atomic force microscopy (AFM) and rheometry can be used to measure the mechanical properties of the ECM at different stages of healing, providing insights into how rehabilitation exercises can be designed to modulate ECM remodeling and improve ligament strength