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Description

Cells were washed with phosphate buffer solution (Biosharp, China), and cells were digested with 0.25% trypsin (Gibco, ThermoFisher, USA)

Inductively coupled plasma mass spectrometry (ICP-MS) or atomic absorption spectroscopy (AAS) should confirm a 1:1 Cu:peptide stoichiometry

F.GordonJ

Molecules 20 , 4831 (2020)

These chemical mediators can affect multiple organ systems including the respiratory, circulatory, integumentary, digestive, muscular and nervous system