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For body use, you can leave it on for 3 to 5 minutes before rinsing

After centrifugation and removal of the supernatant, cells were subjected to hypotonic treatment with a solution of 0.075 M KCl for 30 min at 37 C, followed by fixation in a 3:1 methanol to acetic acid solution

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Non-HDL cholesterol refers to the total cholesterol minus HDL cholesterol

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