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After a noticeable improvement in skin tone, the frequency is reduced to once a week for maintenance

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Secondary metabolic markers include fasting glucose, insulin sensitivity via HOMA-IR or glucose tolerance testing, body composition changes (DEXA scan or MRI for fat mass and lean mass distribution), mitochondrial respiration capacity via Seahorse analyzer, and serum markers like HbA1c for long-term glucose control

Our study showed that CuCl 2 and Cu(OAc) 2 had significantly upregulated HaCaT cell expression of IL-1 and IL-8 at both the gene level and protein level