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After 72 h, cells were fixed in 4% PFA for 45 min and then stained with DAPI for 15 min

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Potential clinical signs include: Vomiting (more than twice a month) Diarrhea (lasting more than three weeks or intermittently over one month or more) Weight loss The inflammation is suspected to be caused by an overreaction of the immune system to intestinal contents that are usually well-tolerated, such as certain foods or normal bacteria, and tends to improve in response to diet changes, antibiotics, or steroids

Aging Cell 6, 111119.10.1111/j.1474-9726.2006.00266.x 77 ParkD.JonesK

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