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Cycling conditions were as follows: 50 C and 95 C both for 2 minutes each prior to 40 cycles of 95 C 15 seconds denaturation, 60 C 30 seconds annealing and 72 C also 30 seconds for primer extension, generating a 193 or a 195 bp amplicon for Enterobacteriaceae or 16S rRNA gene, respectively

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