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For TP53-R72P overexpression experiments fully differentiated (day 7) C3H10T1/2 CrispR/Cas9 p53 KO adipocytes were detached using trypsin (0.25%)/collagenase (0.5 mg/ml), washed with PBS and resuspended in electroporation buffer R (Neon Transfection system, MKP1096, Invitrogen) to 6 10 5 cells per 10 l

The challenge

Purification of a human cytochrome P-450 isozyme catalyzing lanosterol 14 alpha-demethylation

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