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At this stage, epigenetic regulatory mechanisms stabilize the transcriptional activity of hypoxia-inducible factors, upregulate the expression of lipid metabolism enzymes, and significantly enhance cellular membrane sensitivity to oxidative stress (176)

Cells were harvested, and cell numbers were determined, lysed by using liquid nitrogen and 37 C for multiple rapid freeze-thaw cycles, and centrifugated at 10,000 g , 4 C for 10 min