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Controlled supplementation and feeding studies have quantified SFN and its mercapturic-acid pathway metabolites (SFNGSH, SFNCys, SFNNAC) in plasma and urine using validated LCMS/MS methods, enabling reliable PK characterization and PD linkage to Nrf2 target gene induction (HO-1, NQO1) in peripheral blood cells

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Due to the lipid-based nature of these putative ligands, we reasoned that nutrient replenishment would restore their intracellular abundance, thereby inactivating NHR-49