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Another study by Xie et al showed that the high-cholesterol diet induced time-dependent alterations in endothelial-dependent and -independent vascular responses, endothelial cell density, smooth muscle-to-collagen ratio, selective phospho-endothelial NOS-Ser1177 expression, and cGMP levels in the penis of mouse model [131]

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A meta-analysis that included data from eight randomized controlled trials with a total of 327 participants with PCOS found reduced-carbohydrate diets, most lowering carbohydrate intake to 4045% of daily calories versus 5560% of calories in control diets, reduced BMI, insulin resistance, and total and LDL-cholesterol levels

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2.9 DLAT oligomerization analysis Protein samples were prepared with non-reducing loading buffer (Beyotime, China) and minimally heated (70C, 5 min)