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D.HannunY

To generate 3xFLAG-mCherry-CTD, DNA fragments encoding mCherry (digested with Hind III and Eco RI) and the AAGAB CTD (amino acids 258 to 315, digested with Eco RI and Xba I) were subcloned into the Hind III and Xba I sites of the p3xFLAG-7.1 vector

Contrastingly, in adults, mutations are often identified in just one of the two alleles, hinting at a haploinsufficiency mechanism

Probiotics can restore the balance of the gut microbiota, reduce intestinal permeability, and suppress systemic inflammation, thereby indirectly protecting the BBB and the brain from the effects of I/R injury (Zhou et al., 2023)