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The positive transformants were selected on YPD plates with addition of antibiotics geneticin (G418, 4 mg/mL) or hygromycin B (HygB, 1 mg/mL) and verified by DNA sequencing after the integrated gene fragments were amplified by PCR using the corresponding primers and the genomic DNA templates

For SubQ or IM injection, only research-grade lyophilized peptide reconstituted with bacteriostatic water should be used

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GHK-Cu for Research Use Table of Contents GHK-Cu is a copper-binding tripeptide studied in extracellular matrix, collagen and tissue-remodelling research

doi: 10.1038/s41574-022-00711-5