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Description

10.1097/JSA.0000000000000387 211 WuY

The antioxidant selenium is required during this conversion process, its role being to neutralize the toxic build-up of hydrogen peroxide (H2O2) which is produced as a by-product of the hormone conversion

The FMOC-derivatization process involved mixing 10 L of the aqueous extract with 90 L 13 C- and 15 N-labeled amino acid standard solution (20 g per mL) and 100 L borate buffer (0.8 M, pH 10)