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Study of the changes of 10 flavonoids in crude and processed aurantii fructus (zhiqiao) by ultrahigh-performance liquid chromatography and high-resolution tandem mass spectrometry

Two clinically studied peptides

Additionally, the protein expression of autophagic markers, LC3 and p62, and adenosine monophosphate activated protein kinase (AMPK) were also assessed in aortic homogenates

Cells were transfected with GSTO1 siRNA (siGSTO1-1, siGSTO1-2 and siGSTO1-3) or scrambled control siRNA (siSCRAM) using Lipofectamine RNAiMAX (Invitrogen, Thermo Fisher Scientific) following the manufacturers instructions