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The cell powder was resuspended with lysis buffer (20 mM HEPESKOH pH 7.4, 100 mM KOAc, 2 mM Mg(OAc) 2 , 0.5 mM DTT, 1 mM PMSF and one tablet/10 mL complete mini EDTA-free (Roche)) to prepare the crude extracts

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It is particularly important because Bells palsy is linked to the reactivation of the Herpes Simplex Virus- Type 1(HSV-1), which relies on arginine to replicate and reproduce