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Next, the nucleolus fraction was obtained by sonication of the purified nuclei fraction using a S-250D sonicator (Branson Ultrasonic S.A.) with a 1/8'' (3.2 mm) microtip at 30% power of the device in three cycles/sequences (10-s sonication and 10-s pause)

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They modulate intestinal tissue dendritic cells to produce IL-10, decrease IFN- release by intestinal T-cells and decrease LPS-induced production of the proinflammatory IL-12 [119]

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