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Immunofluorescence studies Cryosections (10 m thickness) prepared from the 3D-collagen hydrogel encapsulated with GMSCs were permeabilized in 0.5% Triton X100 for 20 min and blocked with 2.5% goat serum in PBS at room temperature for 1 h

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, 2 .In certain embodiments, the methods herein include treating obesity by orally administering a GLP-1 peptide analog (eg, SEQ ID NO:2 or its ammonium acetate salt (MEDI7219)) to a subject in need thereof

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