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The electroporation cuvette was inserted into the Gene Pulser Xcell Total System (Bio-Rad, Hercules, CA, USA) and electroporated under the following conditions: pulse type: square waveforms

The unique ability of the BBB to sequester GSH from the blood circulation through its Na + -dependent GSH transporter provides a solid foundation for using the transporter as a target to develop brain-targeting drug delivery systems

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Alternatively, the yeast may be grown under continuous culture conditions, allowing for continuous harvesting of culture medium and therefore recovery of secreted glutathione