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Moreover, the genomic and transcriptional signatures of sub-cultured PDXs closely mirror those of the original PDX [176]

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The precleared lysates were incubated with antibodies against SNARE proteins (Syn4, SNAP-23, VAMP2, and VAMP8) for 2 h at 4C for binding, and then the antibodyantigen complexes were precipitated by incubation with a protein G slurry overnight with constant shaking

Surgical trauma initiates complex biological processes, including inflammation, cellular repair, protein synthesis, and tissue remodeling

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