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Aliquots of 300 ul of the supernatant were transferred to fresh tubes for further analysis, while the remaining supernatant fluid was discarded and the protein pellet resuspended in 200 ul of 1 N NaOH and analyzed for protein quantification using the BioRad DC Assay with BSA as a standard

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Moreover, ATP-binding cassette transporter A1 (ABCA1) is also upregulated by LXR in macrophages, and since apoC-II, as well as apoE, apoC-I, and apoC-IV are all able to act as acceptors for cholesterol efflux from macrophages 73 , the coordinate upregulation of ABCA1 and this gene cluster may enhance cholesterol efflux from macrophages, including foam cells in the arterial wall 55

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Total, Bioavailable, and Non-bioavailable Nutrient Fractions The total content of Ca, Mg, P, K, Cu, Fe, Mn, Mo, Zn, and Na was determined following the alkaline melting method reported by Jones and Dreher (1996)