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For the 3.2 mg dose used here, 2.5 mL of BAC water in an 80 mg vial produces 25 draws of 0.1 mL, or 10 units

All samples had their RNA quantified by fluorometry using the Qubit RNA HS Assay Kit (#Q32852) and the Qubit 2.0 fluorometer (Invitrogen, Carlsbad, CA)