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All samples were analyzed immediately, or within the first 24 h, after being stored in a refrigerator below 5C to prevent degradation and microbial activity

Then, samples were centrifugated at 15,000 g for 10 min at 4 C, and the supernatant, containing the extracted D-/L-lactate, was moved into clean tubes

it possesses redox properties that transport selenium to tissues while simultaneously protecting endothelial function [66, 67]

One of the stages of wound healing is inflammation as a part of clearing out damaged tissue 264

The reaction was initiated by adding 100 L of 2.5 mM nicotinamide adenine dinucleotide phosphate (NADPH), and after mixing thoroughly, the rate of absorption change at 412 nm was measured spectrophotometrically