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The resulting cDNA was subjected to quantitative real-time PCR using the Power SYBR Green PCR Master Mix (Applied Biosystems) according to the manufacturers instructions

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In the following sections, we will review the nonFDA-approved medications that have evidence for, and that have been trialed in, post-TBI impairments in attention and concentration and categorize them into tiers based on the available evidence for their efficacy (Figure 1, Figure 2, and Table 1)

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For example, an siRNA having a "sequence sufficiently complementary to a target mRNA sequence to direct target-specific RNA interference (RNAi)" means that the siRNA has a sequence sufficient to trigger destruction of the target mRNA by the RNAi mechanism or process

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