glutathione vs glutathione with vitamin c 10 Cooperation of vitamins and E. R • initiator of a Vitamin C vs Glutathione —

Finally, 100-L of stop solution was added to each well and the optical density (OD) was recorded at 450 nm using a Biotek ELX 800 microplate reader

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Proteins were then precipitated by addition of 500 l methanol (pre-cooled at 80 C), 400 l LCMS-grade water, and 100 l chloroform, and the mixture was vortexed and centrifuged (10,000 g, 10 min, 4C) to afford a protein disc at the interface of CHCl3 and aqueous layers