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Fosmid library construction and sequencing The fosmid library (GRVF) was constructed from sheared genomic DNA and pKS300 cloning vector

Diseases associated with iron overload Iron overload may arise from genetic predispositions, such as hereditary hemochromatosis [25] or ferroportin disease [26], as well as from acquired factors, including repeated red blood cell (RBC) transfusions due to anemia, increased intestinal iron absorption and ineffective erythropoiesis [27]