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To confuse the issue whether opioids could induce liver necrosis, a very recent case report of a middle-aged man with a chronic history of drug abuse who presented, after an overdose of cocaine and heroin, with acute liver failure, renews interest in opioid hepatotoxicity (Dolkar et al., 2022)

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Studies have shown that VIC is subject to zero-order to first-order pharmacokinetics, making it difficult to predict its possible effects, especially when high doses are administered [24,63,64]

Media extracts were incubated at -80 C for at least 15 minutes before being centrifuged at 15,000g for 20 minutes