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83,84 The BTB also, presents a challenge for BCBM treatment, displaying non-uniform permeability and active efflux of molecules

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Beads were washed three times in cold KPBS, then 10% of bead volume was lysed with 1% Triton buffer for protein extracts and the remaining 90% was extracted in 80% methanol containing heavy labeled amino acid standards (Cambridge Isotope Labs) on a rotator shaker for 10min at 4C