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Resveratrol protects primary rat hepatocytes against oxidative stress damage: Activation of the Nrf2 transcription factor and augmented activities of antioxidant enzymes

Reprod Biol Endocrinol 7 , 7 (2009)

The cell lysates were centrifuged at 12,000 g for 10 minutes and the supernatant was mixed with the FLAG antibody overnight at 4C on a shaker, before incubation with prewashed protein A/G agarose beads at 25C for 4 hours