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doi: 10.3389/fimmu.2025.1709354 Received 20 September 2025 Revised 05 November 2025 Accepted 10 November 2025 Published 28 November 2025 Volume 16 - 2025 Edited by Uzma Saqib, Devi Ahilya University, India Reviewed by Dongcheng Liu, Jinan University, China Yao Tong, University of California, San Francisco, United States Updates Copyright 2025 Wei, Lin, Li, Liu, Yang, Chen, Han, Wang, Qian, Ju and Zheng

doi: 10.1016/s1357-2725(98)00015-6

An in situ brain perfusion technique to study cerebrovascular transport in the rat

For AtSNX1 primary antibody, AtSNXl cDNA was cloned into the pET28a plasmid, which incorporates an N-terminal histidine (His) tag into the expressed protein