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Based on different bleeding sites, hemorrhagic stroke can be generally divided into intracerebral hemorrhage (ICH) and subarachnoid hemorrhage (SAH), whose pathogenesis share some similarity

Immunoprecipitation was performed with Flag agarose M2 affinity beads (Sigma-Aldrich, A2220, 15 l per sample) or with BUD13 antibody (A303-321A) prebound to protein G Dynabeads beads (20 l per sample)