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The sublingual mucosa is also non-keratinized, unlike the hard palate or gums

An update to the 2007 review in Annals of Botany

Cultures were grown until they reached the exponential growth phase (OD 600 = 0.5), filtered through a 5 m diameter pore membrane (Millipore, Inc., Billerica, MA) to remove cell clusters, and then centrifuged and resuspended in fresh media to obtain a single-cell suspension at OD 600 = 0.01

To prepare the tissue sections for processing, they were temporarily deparaffinized, moistened with distilled water, and subjected to inhibition of endogenous peroxidase activity with 10% hydrogen peroxide for 10 min

The cell cycle inhibitor, CDKN2D /p19 INK4d , and NFT molecular signatures significantly overlapped with the senescent neuron population (39)