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EMSA EMSAs were performed by mixing increasing amounts of wt or mutated BRD4 ET (with or without ASXL1 peptide at a 1:1 molar ratio) with 10 nM/lane of 147 bp 601 DNA in 2025 mM Tris pH 7.5, 150 mM NaCl and 5 mM DTT in a 10 L reaction volume

[DOI] [PMC free article] [PubMed] [Google Scholar] 2.Llovet JM, Zucman-Rossi J, Pikarsky E, Sangro B, Schwartz M, Sherman M, Gores G

In this narrative review, we summarized the up-to-date information on various plants/medicinal plant extracts, specific phytochemicals isolated from various plants, their mechanism of action, and how these interact with various targets and help in the control of seizures and treatment of epilepsy

At 0.25mg doses where you are drawing tiny volumes, waste as a percentage is relatively small