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Description

Normally, Nrf2 is sequestered in the cytoplasm by Keap1 (Kelch-like ECH-associated protein 1), a protein that also acts as an adaptor protein for the Cullin 3-dependent E3 ubiquitin ligase to mediate the ubiquitination and degradation of Nrf2

Basahi, T

2019 Apr;10(2):429

The developed precipitate is collected by centrifuging the acetone-protein mixture at 3000 g for 10 min

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