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The ROS family encompasses the superoxide anion (O2-), hydrogen peroxide (H 2 O 2 ), hydroxyl radical (OH), and singlet oxygen (1O2) molecules that exhibit heightened chemical reactivity due to the presence of unpaired electrons in their outer electron configurations (Nakai and Tsuruta, 2021)

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Kornblau, SM, Qiu YH, Womble WF, Cade JS, Jackson CE, Milella M, Konopleva M, Andreeff M

Pietra, S

2.7 Preparation of mitochondria and assay of enzyme activity The enzyme activity of three respiratory-chain complexes was quantified in the mitochondria of L-02 hepatocytes treated with CGA and in untreated cells, as previously described ( 2.8 Quantitative analysis of oxidized and reduced forms of glutathione (GSH) To detect GSH and GSSG (glutathione disulfide), two 200 L sample solutions were prepared